Overview

PRELIMINARY CONFERENCE PROGRAMME

YOUNG DEVELOPMENTAL BIOLOGISTS’ FORUM

From 09:30

Registration

09:45 - 10:00

Welcome and short introduction to the Forum formula

10:00 - 13:00

GROUP I: A crash course on mouse oocytes: how to isolate mouse oocytes and mature them in vitro?

GROUP II: A crash course on chick embryos: how to isolate and manipulate chick embryos?

GROUP III and IV: How to prepare a perfect abstract? – a workshop

13:00 - 14:00

Lunch

14:00 - 17:00

GROUP I and II: How to stop procrastinating and motivate yourself to work? – a workshop

GROUP III: A crash course on mouse oocytes: how to isolate mouse oocytes and mature them in vitro?

GROUP IV: A crash course on chick embryos: how to isolate and manipulate chick embryos?

17:00 - 19:00

Young researchers’ session pt. I
Chairs: Anna Piliszek and Roberto de la Fuente (Institute of Genetics and Animal Biotechnology PAS, Jastrzębiec, Poland)

10 min for a talk + 3 min for Q&A

1. The presence and effect of hydrogen sulfide producing enzymes in boar spermatozoa during capacitation and acrosome reaction.
Aneta Pilsová (Czech University of Life Sciences, Prague, Czech Republic)

2. Assessing blastocyst development and cell lineage specification in inter-strain chimeras generated with a mouse model of autism.
Joanna Czyrska (Institute of Genetics and Animal Biotechnology PAS, Jastrzębiec, Poland)

3. Placental gene expression and morphology suggest a peculiar metabolic balance in the BTBR mouse model of autism spectrum disorders.
Maria Pia Viscomi (Institute of Genetics and Animal Biotechnology PAS, Jastrzębiec, Poland)

4. The role of V-ATPASE in phagocytosis of apoptotic cells in mouse blastocysts and in human trophoblast cell line AC-1M88.
Jozef Pisko (Institute of Animal Physiology, Centre of Biosciences SAS, Košice, Slovakia

5. Insight into St14 (matriptase) role during developmental processes.
Barbora Hutečková (Institute of Animal Physiology and Genetics CAS, Brno, Czech Republic; Masaryk University, Brno, Czech Republic)

6. Pharyngeal patterning in the rostral vertebrate head.
Agáta Horáčková (Charles University, Prague, Czech Republic)

7. The effect of T-2 and zearalenone toxins on chicken embryonic development – Early results.
Arnold Toth (Hungarian University of Agriculture and Life Sciences, Gödöllő, Hungary)

8. Investigating the role of YY1 Associated Factor 2 in mouse neural differentiation.
Emese Bélai (Biological Research Centre, Szeged, Hungary; University of Szeged, Szeged, Hungary)

9. Overexpression of R-spondin 3 in the murine ectoderm alters morphogenesis and development of hair follicles.
Szymon Ławicki (University of Warsaw, Warsaw, Poland)

10. Influence of neoadjuvant therapy on fibroblasts migration and chemotaxis in co-culture with autologous adipose-derived stem cells.
Maciej Pancewicz (Medical Univesity of Gdansk, Gdańsk, Poland)

17:00 - 18:30

Developmental biology for everyone – online outreach event
2 x popular science lectures (45 min each)

19:00

Grill party

Venue: Patio of the Faculty of Biology, UW

YOUNG DEVELOPMENTAL BIOLOGISTS’ FORUM

10:00 - 12:00

Young researchers’ session pt. II
Chairs: Anna Ajduk (University of Warsaw, Warsaw, Poland) and Katarzyna Filimonow (Institute of Genetics and Animal Biotechnology PAS, Jastrzębiec, Poland)

10 min for a talk + 3 min for Q&A

1. The influence of in vitro culture and sodium citrate supplementation on the development and differentiation of preimplantation rabbit embryos.
Patrycja Konowrocka (Institute of Genetics and Animal Biotechnology PAS, Jastrzębiec, Poland)

2. Investigating the consequences of Tead1 silencing on epiblast formation in the murine blastocyst.
Dominika Żbikowska (Institute of Genetics and Animal Biotechnology PAS, Jastrzębiec, Poland)

3. Investigating the regulatory role of Tead4 in blastomere positioning and cell fate during mouse preimplantation embryogenesis.
Rebecca Collier (University of South Bohemia, České Budějovice, Czech Republic)

4. Genotype dependent effects of ionizing radiation in zebrafish embryos.
Barnabás Németh (ELI Attosecond Light Pulse Source, Szeged, Hungary)

5. Zebrafish hatching and potassium channels.
Ruchi Prakash Jain (International Institute of Molecular and Cell Biology, Warsaw, Poland)

6. The integration of embryonic gonad cell suspension in guinea fowl.
András Ecker (Hungarian University of Agriculture and Life Sciences, Gödöllő, Hungary)

7. Homeotic transformation of amphibian pharyngeal arches through inhibition of RAR receptors.
Jan Vintr (Charles University, Prague, Czech Republic)

8. Modifying membrane potential alters somitogenesis clock in chick embryo.
Manohara Mahadeva (Institute of Animal Reproduction and Food Research PAS, Olsztyn, Poland)

MAIN CONFERENCE

From 11:00

Registration

12:00 - 13:00

Lunch

13:00 - 13:15

Official opening of the conference

13:15 - 15:15

SESSION I. ORGANOGENESIS
Chair: Marcela Buchtova (Masaryk University and Institute of Animal Physiology and Genetics CAS, Brno, Czech Republic)

13:15 - 14:00

INVITED LECTURE
Differential neural crest gene regulatory subcircuits along the body axis.
Marianne Bronner (California Institute of Technology, Pasadena, USA)

14:00 - 14:15

Towards identification of novel organizers of embryonic development in vertebrates.
Vladimír Soukup (Charles University, Prague, Czech Republic)

14:15 - 14:30

Variation in neural crest development and functional divergence of sox10 duplicates contribute to morphological evolution in teleost fishes.
Aleksandra Marconi (University of Cambridge, Cambridge, UK)

14:30 - 14:45

Comparative analysis of vomeronasal organ development in squamate reptiles.
Paweł Kaczmarek (University of Silesia in Katowice, Katowice, Poland)

14:45 - 15:00

Single-cell sequencing reveals specialized fibroblasts for protecting the skeleton.
Peter Fabian (Masaryk University, Brno, Czech Republic; University of Southern California, Los Angeles, USA)

15:00 - 15:15

Diverse mesenchyme subtypes inform embryonic pancreatic architecture and organization.
Wojciech J. Szlachcic (Adam Mickiewicz University, Poznan, Poland)

15:15 - 15:45

Coffee break

15:45 - 17:45

SESSION II. EARLY EMBRYONIC DEVELOPMENT PT. I
Chair: Marek Maleszewski (University of Warsaw, Warsaw, Poland)

15:45 - 16:30

INVITED LECTURE
Long-standing notions in embryo biology revisited: the cases of zona pellucida and animal pole in mice.
Michele Boiani (Max Planck Institute for Molecular Medicine, Munster, Germany)

16:30 - 16:45

CHK1-CDC25A-CDK1 regulate cell cycle progression in early mouse embryos to protect genome integrity.
David Drutovic (Institute of Animal Physiology and Genetics CAS, Libechov, Czech Republic)

16:45 - 17:00

Interspecies comparison of protein expression and degradation during preimplantation development of mammals.
Tereza Toralová (Institute of Animal Physiology and Genetics CAS, Liběchov, Czech Republic)

17:00 - 17:15

Glutamate and GABA can act as signaling molecules in preimplantation embryos.
Štefan Čikoš (Institute of Animal Physiology, Centre of Biosciences SAS, Košice, Slovakia)

17:15 - 17:30

Spatial positioning of preimplantation mouse embryo cells is regulated by mTORC1 and 7mG-cap dependent translation at the 8- to 16-cell transition.
Alexander W. Bruce (University of South Bohemia, České Budějovice, Czech Republic)

17:30 - 17:45

The RUNT-related transcription factor RUNX1 accompanies primitive endoderm lineage segregation within the ICM during mouse blastocyst formation.
Roberto de la Fuente (Institute of Genetics and Animal Biotechnology PAS, Jastrzębiec, Poland; University of Manchester, Manchester, UK)

17:45 - 18:15

Coffee break

18:15 - 19:15

V4SDB General Assambly

19:15 - 20:15

Poster session (odd numbers)

20:00 - 23:00

Welcome party

Venue: Reception area of the Faculty of Biology, UW

MAIN CONFERENCE

From 09:00

Registration

09:00 - 11:00

SESSION III. CELLULAR DYNAMICS AND MIGRATION IN DEVELOPMENTAL BIOLOGY
Chair: Vitezslav Bryja (Masaryk University, Brno, Czech Republic)

09:00 - 09:45

INVITED LECTURE
Genetic dissection of vertebrate gastrulation.
Lilianna Solnica-Krężel (Washington University School of Medicine in St. Louis, St. Louis, USA)

09:45 - 10:00

Epithelial stratification in the oropharynx of the sterlet sturgeon (Acipenser ruthenus).
Ann Huysseune (Charles University, Prague, Czech Republic)

10:00 - 10:15

BEE-ST Method: 4D-Monitoring of biomineralization dynamics in bones and teeth.
Marcos Gonzalez Lopez (Masaryk University; Brno, Czech Republic)

10:15 - 10:30

Cellular mechanisms driving early development of the mammary gland.
Ewelina Trela (University of Helsinki, Helsinki, Finland; University of Warsaw, Warsaw, Poland)

10:30 - 10:45

Spatial mapping of stromal microenvironment during mammary branching morphogenesis.
Zuzana Sumbalova Koledova (Masaryk University, Brno, Czech Republic)

10:45 - 11:00

The velocity of cytoplasmic movement as a marker of the keratin cytoskeleton quality in embryos.
Agnieszka Walewska (University of Warsaw, Warsaw, Poland)

11:00 - 11:30

Coffee break + group photos

11:30 - 13:30

SESSION IV. GENETIC AND EPIGENETIC CONTROL OF DEVELOPMENTAL PROCESSES
Chair: Peter Vilmos (Biological Research Centre, Szeged, Hungary)

11:30 - 12:15

INVITED LECTURE
RNA silencing pathways in mammalian germ cells.
Petr Svoboda (Institute of Molecular Genetics CAS, Prague, Czech Republic)

12:15 - 12:30

Short stories about long RNAs during early mammalian development.
Joanna W. Jachowicz (Institute of Molecular Biotechnology AAS, Vienna Biocenter, Vienna, Austria)

12:30 - 12:45

Spatio-temporal regulation of maternal mRNAs.
Denisa Jansova (Institute of Animal Physiology and Genetics, CAS, Liběchov, Czech Republic)

12:45 - 13:00

Regulation of embryonic gene expression through chromatin marks and transcriptional condensates.
Adam Kłosin (Nencki Institute of Experimental Biology PAS, Warsaw, Poland)

13:00 - 13:15

The DNA single-strand break repair capacity of the mammalian oocytes and embryos.
Helena Fulka (Institute of Experimental Medicine CAS, Prague, Czech Republic)

13:15 - 13:30

Genomic uracil and dUTP dynamics during early zebrafish development.
Máté Varga (ELTE Eötvös Loránd University, Budapest, Hungary)

13:30 - 14:30

Lunch

13:30 - 14:30

V4SDB steering committee meeting

14:30 - 16:30

SESSION V. STEM CELLS AND REGENERATION
Chair: Maria Anna Ciemerych-Litwinienko (University of Warsaw, Warsaw, Poland)

14:30 - 15:15

INVITED LECTURE
Myogenic cell heterogeneity during development and disease.
Giulio Cossu (University of Manchester, Manchester, UK; San Raffaele Hospital, Milan, Italy)

15:15 - 15:30

Regeneration initiation studied using single cell and spatial transcriptomics.
Radek Sindelka (Institute of Biotechnology, Vestec, Czech Republic)

15:30 - 15:45

Fluctuation of stem cell niche as a source of tissue adaptability.
Jan Krivanek (Masaryk University, Brno, Czech Republic)

15:45 - 16:00

Upregulation of Mitophagy by JAK-STAT Signaling Enables Stem Cell Regeneration in Drosophila male germline.
Tibor Kovács (ELTE Eötvös Loránd University, Budapest, Hungary)

16:00 - 16:15

Regenerative and Immunomodulating Potential of Sertoli Cells.
Vladimír Krylov (Charles University, Prague, Czech Republic)

16:15 - 16:30

Influence of neoadjuvant therapy on fibroblasts migration and chemotaxis in co-culture with autologous adipose-derived stem cells.
Aneta Skoniecka (Medical Univesity of Gdansk, Gdańsk, Poland)

16:30 - 17:00

Coffee break

17:00 - 18:00

Poster session (even numbers)

20:00

Gala dinner
Awards for the best oral and poster presentations

Venue: Sound Garden Hotel

MAIN CONFERENCE

From 09:00

Registration

09:00 - 11:00

SESSION VI. EARLY EMBRYONIC DEVELOPMENT PT. II
Chair: Alexander W. Bruce (University of South Bohemia, České Budějovice, Czech Republic)

09:00 - 09:45

INVITED LECTURE
From trash bin to gold mine: implications of extracellular vesicles in early pregnancy.
Monika Kaczmarek (Institute of Animal Reproduction and Food Research PAS, Olsztyn, Poland)

09:45 - 10:00

ATP-Citrate Lyase, a key metabolic player in sheep blastocyst development.
Margherita Moncada (University of Teramo, Teramo, Italy)

10:00 - 10:15

Bovine interspecies embryonic chimera with wisent somatic cells.
Agnieszka Bernat (Institute of Genetics and Animal Biotechnology PAS, Jastrzębiec, Poland; Intercollegiate Faculty of Biotechnology UG&MUG, Gdańsk, Poland)

10:15 - 10:30

X-chromosome inactivation in mouse embryos and embryonic tissue.
Martin Urbán (Hungarian University of Agriculture and Life Sciences, Gödöllő, Hungary)

10:30 - 10:45

At what stage of post-implantation development does the regulation of cell number occur in mouse chimaeric embryos?
Marek Maleszewski (University of Warsaw, Warsaw, Poland)

10:45 - 11:00

Maternal RNA localization during early axolotl development.
Kateřina Šimková (Institute of Biotechnology CAS, Prague, Czech Republic)

11:00 - 11:30

Coffee break

11:30 - 13:30

SESSION VII. IN VITRO MODELS IN DEVELOPMENT AND DISEASE
Chair: Berenika Plusa (University of Manchester, Manchester, UK)

11:30 - 12:15

INVITED LECTURE
Gastruloids: an ESC based model for mammalian gastrulation and body plan engineering.
Alfonso Martinez Arias (Pompeu Fabra University, Barcelona, Spain)

12:15 - 12:30

Myogenic differentiation of pluripotent stem cells.
Maria Anna Ciemerych-Litwinienko (University of Warsaw, Warsaw, Poland)

12:30 - 12:45

Novel mechanisms and potential treatment of Duchenne muscular dystrophy-associated cardiomyopathy.
Józef Dulak (Jagiellonian University, Krakow, Poland)

12:45 - 13:00

Role of the epigenetic regulator RYBP in mammalian cardiac lineage commitment.
Lilla Kókity (Institute of Genetics, Biological Research Centre, ELKH, Szeged, Hungary; University of Szeged, Szeged, Hungary)

13:00 - 13:15

When bad things happen to good cells: the role of autophagy in the development of neonatal glial cells.
Paulina Gębala (Mossakowski Medical Research Institute Polish Academy of Sciences, Warsaw, Poland)

13:15 - 13:30

SPOCK2 via MMP2 controls proliferation and function expansion of human early pancreatic β-cells.
Katarzyna Błaszczyk (Adam Mickiewicz University, Poznan, Poland)

13:30 - 14:00

Closing remarks

14:00 - 15:00

Lunch

PRELIMINARY CONFERENCE PROGRAMME

Venue: Patio of the Faculty of Biology, UW

Venue: Reception area of the Faculty of Biology, UW

Venue: Sound Garden Hotel